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Valiant Co Ltd chicken embryo extract
Chicken Embryo Extract, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+embryo+extract/Chicken+embryo+extract/pmc13049958-318-40-43
Average 95 stars, based on 115 article reviews
chicken embryo extract - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Centrifugation:

Article Title: Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis
Article Snippet: Briefly, dissected hind limb muscles were minced and enzymatically digested with collagenase type II (500 U/mL, Sigma), collagenase D (1.5 U/mL, Sigma), dispase II (2.5 U/mL, Sigma) and CaCl 2 (2.5 mM, Roth) for 1h at 37°C. .. After centrifugation (300× g , 5 min), the suspension containing small pieces of the muscle tissue was resuspended in fresh proliferation medium, containing high-glucose DMEM (4.5 g/L glucose, PAN-Biotech), 20% fetal bovine serum (FBS, PAN-Biotech), 10% horse serum (PAN-Biotech), 0.5% chicken embryo extract (MP biomedicals), 2.5 ng/mL basic fibroblast growth factor (Sigma) and 1% penicillin/streptomycin (PAN-Biotech), and seeded on matrigel-coated (Corning, New York, USA) petri dishes. ..

Article Title: Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis.
Article Snippet: Briefly, dissected hind limb muscles were minced and enzymatically digested with collagenase type II (500 U/mL, Sigma), collagenase D (1.5 U/mL, Sigma), dispase II (2.5 U/mL, Sigma) and CaCl2 (2.5 mM, Roth) for 1h at 37°C. .. After centrifugation (300 x g, 5 min), the suspension containing small pieces of the muscle tissue was resuspended in fresh proliferation medium, containing high- Jo urn al Pr e-p r o f 22 glucose DMEM (4.5 g/L glucose, PAN-Biotech), 20% fetal bovine serum (FBS, PAN-Biotech), 10% horse serum (PAN-Biotech), 0.5% chicken embryo extract (MP biomedicals), 2.5 ng/mL basic fibroblast growth factor (Sigma) and 1% penicillin/streptomycin (PAN-Biotech), and seeded on matrigel-coated (Corning, New York, USA) petri dishes. ..

Suspension:

Article Title: Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis
Article Snippet: Briefly, dissected hind limb muscles were minced and enzymatically digested with collagenase type II (500 U/mL, Sigma), collagenase D (1.5 U/mL, Sigma), dispase II (2.5 U/mL, Sigma) and CaCl 2 (2.5 mM, Roth) for 1h at 37°C. .. After centrifugation (300× g , 5 min), the suspension containing small pieces of the muscle tissue was resuspended in fresh proliferation medium, containing high-glucose DMEM (4.5 g/L glucose, PAN-Biotech), 20% fetal bovine serum (FBS, PAN-Biotech), 10% horse serum (PAN-Biotech), 0.5% chicken embryo extract (MP biomedicals), 2.5 ng/mL basic fibroblast growth factor (Sigma) and 1% penicillin/streptomycin (PAN-Biotech), and seeded on matrigel-coated (Corning, New York, USA) petri dishes. ..

Article Title: Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis.
Article Snippet: Briefly, dissected hind limb muscles were minced and enzymatically digested with collagenase type II (500 U/mL, Sigma), collagenase D (1.5 U/mL, Sigma), dispase II (2.5 U/mL, Sigma) and CaCl2 (2.5 mM, Roth) for 1h at 37°C. .. After centrifugation (300 x g, 5 min), the suspension containing small pieces of the muscle tissue was resuspended in fresh proliferation medium, containing high- Jo urn al Pr e-p r o f 22 glucose DMEM (4.5 g/L glucose, PAN-Biotech), 20% fetal bovine serum (FBS, PAN-Biotech), 10% horse serum (PAN-Biotech), 0.5% chicken embryo extract (MP biomedicals), 2.5 ng/mL basic fibroblast growth factor (Sigma) and 1% penicillin/streptomycin (PAN-Biotech), and seeded on matrigel-coated (Corning, New York, USA) petri dishes. ..

Immunostaining:

Article Title: Transient hypoxia followed by progressive reoxygenation is required for muscle repair.
Article Snippet: .. Single myofibers were either immediately fixed in 4% PFA for 10 min before immunostaining or maintained for 72 h in myofiber growth medium containing 4.5 g/L glucose 1x DMEM-GlutamaxTM-1 (GibcoTM; #31966), 1% Penicillin/Streptomycin (GibcoTM; #15140), 20% fetal bovine serum (FBS; GibcoTM; #10270) and 1% chicken embryo extract (CEE; MP Biomedical; CE650-J) at 37 °C and 5%CO2. ..

Article Title: Transient hypoxia followed by progressive reoxygenation is required for muscle repair
Article Snippet: .. Single myofibers were either immediately fixed in 4% PFA for 10 min before immunostaining or maintained for 72 h in myofiber growth medium containing 4.5 g/L glucose 1x DMEM-Glutamax TM -1 (Gibco TM ; #31966), 1% Penicillin/Streptomycin (Gibco TM ; #15140), 20% fetal bovine serum (FBS; Gibco TM ; #10270) and 1% chicken embryo extract (CEE; MP Biomedical; CE-650-J) at 37 °C and 5%CO 2 . ..

Article Title: Loss of cell-autonomously secreted laminin-α2 drives muscle stem cell dysfunction in LAMA2-related muscular dystrophy
Article Snippet: .. Once a sufficient number of fibers were obtained, they were individually transferred to dishes containing culture media (20% FBS (PAN-Biotech), 1% Chicken Embryo Extract (Mpbio) and 2.5 ng/ml bFGF (Gibco) in isolation media) and incubated at 37 °C and 5% CO 2 until collection for immunostaining (see Immunostaining of single fibers). ..

Modification:

Article Title: Alginate-encapsulated muscle-derived stem cell spheroids promote muscle regeneration in a murine model of volumetric muscle loss
Article Snippet: .. Unless otherwise specified, MDSC were cultivated in Proliferation Medium (PM), constituted by Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Inc., United States) supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher), 10% Horse Serum (HS, Thermo Fisher), 0,5% Chicken Embryo Extract (MP Biomedicals, United States) and 1% Penicillin/Streptomycin (Thermo Fisher). ..

Article Title: The HIF-1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation.
Article Snippet: Pre- plated cells were cultured in low- glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM- HG with 5% horse serum (HS, Merck).

Article Title: The HIF‐1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation
Article Snippet: Pre‐plated cells were cultured in low‐glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM‐HG with 5% horse serum (HS, Merck).

Isolation:

Article Title: The HIF-1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation.
Article Snippet: Pre- plated cells were cultured in low- glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM- HG with 5% horse serum (HS, Merck).

Article Title: Loss of cell-autonomously secreted laminin-α2 drives muscle stem cell dysfunction in LAMA2-related muscular dystrophy
Article Snippet: .. Once a sufficient number of fibers were obtained, they were individually transferred to dishes containing culture media (20% FBS (PAN-Biotech), 1% Chicken Embryo Extract (Mpbio) and 2.5 ng/ml bFGF (Gibco) in isolation media) and incubated at 37 °C and 5% CO 2 until collection for immunostaining (see Immunostaining of single fibers). ..

Article Title: The HIF‐1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation
Article Snippet: Pre‐plated cells were cultured in low‐glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM‐HG with 5% horse serum (HS, Merck).

Cell Culture:

Article Title: The HIF-1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation.
Article Snippet: Pre- plated cells were cultured in low- glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM- HG with 5% horse serum (HS, Merck).

Article Title: The HIF‐1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation
Article Snippet: Pre‐plated cells were cultured in low‐glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM‐HG with 5% horse serum (HS, Merck).

Concentration Assay:

Article Title: The HIF-1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation.
Article Snippet: Pre- plated cells were cultured in low- glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM- HG with 5% horse serum (HS, Merck).

Article Title: The HIF‐1α Pathway Regulates Satellite Cell Fate During Aging Through Histone Lactylation
Article Snippet: Pre‐plated cells were cultured in low‐glucose DMEM (1 g/L; Merck) supplemented with 10% FBS (Merck), 1% glutamine (Merck), and 1% penicillin/streptomycin (Euroclone), and were used as skeletal muscle fibroblasts to assess SC preparation contamination. .. Isolated SCs were cultured in growth medium (GM) consisting of Dulbecco's Modified Eagle's Medium (DMEM, Merck) with high glucose concentration (HG, 4.5 g/L) supplemented with 20% fetal bovine serum (FBS, Merck), 10% horse serum (HS, Merck), 1% glutamine (Merck), 1% penicillin/streptomycin (Euroclone), and 1% Chicken Embryo Extract (CEE, MP Biomedicals). .. The differentiation medium (DM) consisted of DMEM‐HG with 5% horse serum (HS, Merck).

Incubation:

Article Title: Loss of cell-autonomously secreted laminin-α2 drives muscle stem cell dysfunction in LAMA2-related muscular dystrophy
Article Snippet: .. Once a sufficient number of fibers were obtained, they were individually transferred to dishes containing culture media (20% FBS (PAN-Biotech), 1% Chicken Embryo Extract (Mpbio) and 2.5 ng/ml bFGF (Gibco) in isolation media) and incubated at 37 °C and 5% CO 2 until collection for immunostaining (see Immunostaining of single fibers). ..



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